BIOCHM 623
Spring 2007

1. Lecture 1. January 28. Evolutionary and Genetic Origin of Protein Sequences.

Interesting article on BLAST.

2. Paper discussion. January 30. Resurrecting the ancestral enzymatic role of a modulatory subunit. 1) J. Biol. Chem. 2005. 280, 10189. 2) Review.

1) PDI; 2) ADP-Glc PPase

3. Lecture 2. February 1.Physical interactions that determine the properties of proteins. Secondary and tertiary structure.

Creighton Chapter 4

4. Lecture 3. February 4. Protein Domains, Motifs, and Quaternary Structure.

5. Paper discussion. February 6. A loop network within the anthrax toxin pore positions the Phe clamp in an Active conformation. 1) PNAS 2006. 103, 9802. 2) Review 3) Powerpoint slides

6. Lecture 4. February 8. Spectral Methods of Characterizing Protein Conformation and Conformational Changes. Absorbance Spectroscopy.

7. Lecture 5. February 11. February 15. Spectral Methods of Characterizing Protein Conformation and Conformational Changes. CD and Fluorescence spectroscopy

References

8. Paper discussion. February 13. Scanning the membrane-bound conformation of helix 1 in the colicin E1 channel domain by site-directed fluorescence labeling. 1) J. Biol. Chem. 2006. 281, 885. 2) Review 3) Power Point slides

9. Lecture 6 . February 15. Intramolecular interactions and Interaction with other molecules: cooperativity and binding.

Creighton Chapter 8

10. Review Session. February 19 (Tuesday)

11. Exam 1. February 20. 9.05 AM to 11AM in Class

12. Lecture 7 . Enzyme kinetics: catalysis. (*) Study guide.

13. Lecture 8. February 25. Exam 3 revision. Catalysis and control of protein function.

14. Paper Discussion. March 5. TBA

15. Lecture 9 . February 29.Control of protein functions. Movie on signaling.

16. Lecture 10. March 3.Lipids, lipid mixtures, and membranes.

17. Paper Discussion. February 27. Protein Kinase C as a Molecular Machine for Decoding Calcium and Diacylglycerol Signals. 1) Cell. 1998; 95:307-18. 2) Review 3) Slides

1) Movie1 PKC-GFP goes to the membrane 2) Movie2 PKC membrane binding cartoon

18. Lecture 11. March 7. Protein-lipid interactions and the lipid rafts hypothesis.

Papers: 1) Protein domains in neuronal signal transduction. 2) Lipids rafts: Elusive or Illusive?

3) Jacobson et al 2007. Nat. Cell Biol. 9, 7-14. Review on Rafts.

a) Movie. b) Raft definition.

19. Review Session. March 10.

20.Exam 2. March 12. 9.30 AM to 11.30 AM in room TBA

21.. March 14. Guest Lecture. TBA.

HAVE A GREAT Spring Break!!!!

Free online books: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Books