Laboratory Equipment
Dr. Clarkson's laboratory is a newly renovated 1,900 sq ft space.
It has separate areas for muscle imaging, biochemistry procedures,
muscle sectioning, and gel electrophoresis. It also has a separate
space for subject muscle function testing and Doppler/ultrasound.
The biochemistry space includes a fume hood and wet lab.
The laboratory is equipped with a MICROM HM 505E Cryostat (Richard Allan Scientific, Kalamazoo, MI), a Nikon Eclipse E400 Phase Contrast Fluorescent Microscope (Nikon Corporation, Tokyo, Japan) equipped with a Spot insight QE camera and EclipseNET software, version 1.16 for image analyses, EC135-90 power supply with gel electrophoresis and transfer systems (Owl Separation Systems, Portsmouth, NH), 2 spectrophotometers at UV and visible wavelengths, microplate photometers (Thermo Labsystems), waterbaths, distiller, IEC MicroMax RF refrigerated centrifuge, Sorvall Legend RT refrigerated centrifuge, ultrasonic cell disruptor (Misonix, Farmingdale, NY), Acuson 128 XP Ultrasound with 5.0 and 7.0 Mhz linear array transducer and a -80 C Forma Scientific Freezer.
The laboratory has recently added equipment for RNA isolation and protein isolation to analyze the molecular processes of muscle hypertrophy and atrophy. The lab is equipped with a Laminar Flow PCR Workstation (Fisher Scientific), Isotemp 145D Digital Dry-Bath Incubator (Fisher Scientific), ND-1000 UV-Vis Spectrophotometer (Nanodrop Technologies), Dual Light Transilluminator (FOTODYNE), Mini Darkroom Hood (FOTODYNE), Hybridization Incubator (Fisher Scientific), Olympus Digital Camera (CCD camera with 8-bit standard), and Phoretix TotalLab gel analysis software (FOTODYNE).