We use and develop tools for measuring and manipulating single molecules. Our goal is a better understanding of how biological molecules and molecular complexes behave in confining and complex environments, up to and including living cells. Using physical principles and optical tools, our research is motivated by a desire to understand how individual biomolecular components work together to drive the behavior of living systems.
We often make use of high-quantum-yield fluorophores (dye molecules), which are attached to the biomolecule under study. These bright fluorophores act as nanoscopic probes of their local environment, their brightness and spectrum reporting, for example, on changes in biomolecular structure or dynamics.
It is remarkable that individual molecules can be probed at all, but today it is possible to "see" single molecules with electron, optical, and atomic force microscopies, and to pull on and measure the force exerted by individual molecules using microfabricated cantilevers or optical tweezers. Indeed, single-molecule-sensitive measurements have become ubiquitous since the turn of the millennium, and the exquisite sensitivity they offer is providing valuable new insights into biological and biophysical processes.
Currently there are two classes of systems under study in our lab.
Nucleic Acids. We study the conformational dynamics of small DNA and RNA molecules, in an effort to understand structural changes that are transient, irreversible, or stochastic in nature.
Cellulose is the most abundant polymer on earth, but very little is understood about how plants make it. In collaboration with Tobias Baskin, we study the nanomechanics of cellulose and cellulose synthesis in land plants.
One unique aspect of our in vitro work is the use of nanoscopic emulsion droplets for confining individual molecules. Observing an individual molecule for more than a few milliseconds requires a method to locate and hold the molecule in a detection region. Often this is done by attaching the molecule to a surface, or embedding it in a gel. We use reverse emulsion droplets - smaller than a micron in diameter - as nanoscopic "test tubes" for measurements on single molecules.
To read more about our research, please visit our research page.
For information on current projects, visit my blog. Information on open-source hardware and software developed by us is also linked through the blog.